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. Author manuscript; available in PMC: 2022 Oct 1.

Published in final edited form as: J Mol Cell Cardiol. 2021 Jun 15;159:62–79. doi: 10.1016/j.yjmcc.2021.06.004

Figure 2. — Immune cells were isolated from the infarct tissue 3-day or 7-day after LAD ligation. Cells were seeded on coverslips for two hours before they were fixed with 4%PFA for 10 minutes. RTqPCR was performed using RNAs purified from spleen monocytes (sMo) and cMPs isolated from infarct tissue. A and B. Cells isolated from infarcting myocardium 7-day post MI were labeled with CD68 or CD11b and counter-stained with Tom20 or Actin. C. The focal adhesion structures formed by myeloid integrin CD11b under a higher magnification. D. Percentages of cMPs (CD68+Tom20+or CD11b+Actin+) to total cells (Tom20+ n=211 or Actin+ n=238). Cells were isolated from 4 MI hearts. E. Transcripts of inflammatory cytokines IL1β, TNFα, and IL6 from sMo and cMPs were determined using RT-qPCR. F. Expression of chemokine MCP-1, CCL3, and CCL4 from sMo and cMP. G. Expression of Arg1 and iNOS from the same set of samples as in F. H. The ratio of Arg1 to iNOS. I. Scavenger receptor CD14 expression in sMo and cMPs from sham and MI hearts. J. MMP2 and MMP9 expression from cMP samples as indicated. For RT-qPCR performed in E to J, n=3 to 4 spleens or 10 to 12 MI hearts (some data points were from pooled MI tissues). * p<0.05 ** p<0.01 *** p<0.001.

Figure 2. — Immune cells were isolated from the infarct tissue 3-day or 7-day after LAD ligation. Cells were seeded on coverslips for two hours before they were fixed with 4%PFA for 10 minutes. RTqPCR was performed using RNAs purified from spleen monocytes (sMo) and cMPs isolated from infarct tissue. A and B. Cells isolated from infarcting myocardium 7-day post MI were labeled with CD68 or CD11b and counter-stained with Tom20 or Actin. C. The focal adhesion structures formed by myeloid integrin CD11b under a higher magnification. D. Percentages of cMPs (CD68+Tom20+or CD11b+Actin+) to total cells (Tom20+ n=211 or Actin+ n=238). Cells were isolated from 4 MI hearts. E. Transcripts of inflammatory cytokines IL1β, TNFα, and IL6 from sMo and cMPs were determined using RT-qPCR. F. Expression of chemokine MCP-1, CCL3, and CCL4 from sMo and cMP. G. Expression of Arg1 and iNOS from the same set of samples as in F. H. The ratio of Arg1 to iNOS. I. Scavenger receptor CD14 expression in sMo and cMPs from sham and MI hearts. J. MMP2 and MMP9 expression from cMP samples as indicated. For RT-qPCR performed in E to J, n=3 to 4 spleens or 10 to 12 MI hearts (some data points were from pooled MI tissues). * p<0.05 ** p<0.01 *** p<0.001.