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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: J Mol Cell Cardiol. 2021 Jun 15;159:62–79. doi: 10.1016/j.yjmcc.2021.06.004

Figure 3.

Figure 3.

Myocardial infarction was generated by LAD ligation. Twenty four hours after the procedure, mice were randomly divided into groups that received subcutaneous injection of DMSO or rapamycin at 0.25mg/kg/day. Hearts were harvested either 3-day or 7-day post MI procedure, resulting a total of 2-day or 6-day injection respectively. A. A diagram of the experimental design. B. Protein samples from sham or infarct tissue (7-day post MI) were subjected to immunoblot analysis to detect p-S6 to assess the effectiveness of rapamycin. n=2 hearts from each group. C. Cross sections from 1-week MI hearts were stained with Trichrome C (TC) or Picrosirius red (PSR). Infarct area is estimated using ImageJ software. The percentages of IA (ischemic area) to LV (left ventricle) were calculated. D. Gravimetric data reflecting cardiac remodeling 1-week after LAD ligation. Both heart weight to body weight and heart weight to tibia length ratios were included. n=3 to 7 hearts. E. Expression of cardiac hypertrophy markers in the remote myocardium from mice treated with DMSO or rapamycin. n=3 to 4 hearts. F. αSMA protein levels in the infarct tissues from DMSO- or rapamycin-treated group. n=2 hearts from each group. G. The percentage of infarct to left ventricle was calculated using the tissue wet weight. The infarct tissues were meticulously separated under a dissection microscope. n=10 to 22 MI hearts. H. Survival curves of mouse cohorts receiving either DMSO or rapamycin treatment. n=19 to 33 mice. I. Cardiac function (FS), LV dimensions at the end of diastole (LVID;d) and systole (LVID;s), and heart rate (HR) were measured 4 weeks after MI. Mice were treated with either DMSO or rapamycin for 6 days starting at the second day after LAD ligation. n=4. * p<0.05 ** p<0.01 *** p<0.001.