Figure 4.

Myocardial infarction was generated via LAD ligation. Twenty four hours after the procedure, mice are randomly divided into groups that receive subcutaneous injection of DMSO or rapamycin at 0.25mg/kg/day. Cardiac immune cells were isolated from infarct tissue 3-day or 7-day after MI and prepared for ICC. A. Morphological spectrum of cMPs. Cells were labeled with lysosome marker MAC3 and mitochondria protein Tom20. Representative cMPs from the infarct tissue were classified into cMP1, cMP2, cMP3, and cMP4 based on criteria that separate monocytes from mature macrophages. B. cMPs with morphological criteria of cMP1, cMP2, cMP3, and cMP4 were labeled with CD206. C. Morphology sorting of cMPs isolated from the infarct tissues 3-day and 7-day after LAD ligation and treated with or without DMSO or rapamycin. The percentages of the cMP1, cMP2, cMP3, and cMP4 populations and total cells examined were depicted. D and E. cMPs from DMSO (D) or rapamycin (E) treated hearts were labeled with p-S6 and CD206 antibodies. F. Cryosections of the hearts were labeled with CD68 and MerTK. G. Quantification of MerTK+ cells. n=8 MI hearts for A,B,C,D,E. n=3 MI hearts for F and G. *p<0.05