Fig. 4.

Characterising the strains with engineered acetyl‐CoA synthesis pathways and auxin‐mediated induction of GAL promoters: (A) growth (N = 4) and metabolic profiles (N = 2); (B) maximum specific growth rate (N = 4); (C and D), nerolidol titre (N = 4); (E and F), specific nerolidol production rate (N = 4). Two‐phase flask cultivation on 20 g l⁻¹ glucose was employed, and NAA dissolved in ethanol was added in preculture and at the beginning of the cultivation. Dashed lines in A indicate 24, 72 and 120 h. For C–F, two‐tailed Welch’s t‐test was used for statistical analysis relative to N57BRU: n, P > 0.1; m, P ∈ [.05, 0.1]; s and s′ (calculated from log‐transformed data), P < 0.05. Mean values ± standard deviations are shown.