Table 2.
Plasmids used in this work.
| Plasmid | Features | References |
|---|---|---|
| pRS424 | E. coli/S. cerevisiae shuttle plasmid; 2 μ, TRP1 | Christianson et al. (1992) |
| pRS425 | E. coli/S. cerevisiae shuttle plasmid; 2 μ, LEU2 | Christianson et al. (1992) |
| pJT9R | pRS425: TRPL3<ERG20<PGAL1‐PGAL2>Ac.NES1>TRPL41B | Peng et al. (2017a,2017b) |
| pIR3DH8 | Plasmid used to disrupt GAL80 with URA3 marker | This work |
| pML104 | E. coli/S. cerevisiae shuttle plasmid; 2 μ, URA3, PTDH3‐CRSIPR/Cas9‐TADH1 , PSNR52‐guideRNA‐swaI‐TSUP4 | Laughery et al. (2015) |
| pJCble | pML104; URA3 marker was replaced with ble marker (Goldstein and McCusker, 1999) | This work |
| pIALD2 | pRS424: ALD6 (−125, 40)‐ PADH1 > Lr.pduP> TPDC1 ‐ PSb.GAL2 >Lr. EutE> ALD6 (1054, 1749) | This work |
| pIALD2S | pRS424: ALD6 (−125, 40)> PADH1 > Lr.pduP> TPDC1 ‐ALD6 (1054, 1749) | This work |
| pIALD2E | pRS424: ALD(−125, 40)‐ ALD6(1054, 1749) | This work |
| pIALD2HMGr | pRS424: ALD6(−125, 40)> PSk.GAL1 >Da.mvaA>TPGK1 ‐PADH1 > Lr.pduP> TPDC1 ‐PGAL2 >Lr. EutE‐ALD6(1054, 1749) | This work |
| pIPKA2 | pRS424: GPP1(−220,80)> PENO2 >Lr.xfp>TTPI1 ‐PTDH3 >Mb.pta>TFBA1 ‐GPP1(754, 1400) | This work |
| pIPKAH | pRS424: GPP1(−220,80)> PAg.TEF1>hphMX6(Goldstein and McCusker, 1999)>TAg.TEF1‐PENO2> Lr.xfp>TTPI1‐PTDH3>Mb.pta>TFBA1 ‐GPP1(754, 1400) | This work |
| pJT9R | pRS425: TRPL3<ERG20<PGAL1‐PGAL2>Ac.NES1>TRPL41B | Peng et al. (2017a,2017b) |
| pILGB5A | Yeast integration plasmid; PURA3‐KlURA3‐TAgTEF1‐PGAL2‐yEGFP‐TURA3 | Peng et al. (2015) |
| pJAIDB58T | pILGB5A; PURA3‐KlURA3‐TAgTEF1‐PGAL2‐yEGFP‐ TPGK1‐PACS2‐SKP1 ‐OsTIR1‐TURA3 | This work |
Symbol > or < indicates the direction of the open reading frame.