Table 2.
Comparison of the genes encoding enzymes involved in the sequential oxidation of ethylene glycol (EG) to glyoxylate between P. umsongensis GO16 and P. putida KT2440.
| Gene | Locus | Function | Query cover (%) | Identity AA (%) | Reference gene |
|---|---|---|---|---|---|
| pedI | F6476_19660 | Aldehyde dehydrogenase | 100 | 92 | PP_2680 |
| pedH | F6476_19665 | Quinoprotein ethanol dehydrogenase | 99 | 91 | PP_2679 |
| pedE | F6476_19690 | PQQ dependent dehydrogenase | 100 | 94 | PP_2674 |
| aldB‐I | F6476_27375 | Aldehyde dehydrogenase | 100 | 92 | PP_0545 |
| gcl | F6476_01220 | Carboxylate ligase | 100 | 86 | PP_4297 |
| hyi | F6476_01215 | Hydroxypyruvate isomerase | 100 | 78 | PP_4298 |
| glxR | F6476_01210 | Tartronate reductase | 100 | 86 | PP_4299 |
| ttuD | F6476_01205 | Hydroxypyruvate reductase | 99 | 81 | PP_4300 |
| ttuE | F6476_01200 | Pyruvate kinase | 99 | 79 | PP_4301 |
| glcC | F6476_01190 | Glc operon transcriptional regulator | 98 | 73 | PP_3744 |
| glcD | F6476_01185 | Glycolate oxidase FAD‐linked subunit | 100 | 86 | PP_3745 |
| glcE | F6476_01180 | Glycolate oxidase FAD‐binding subunit | 100 | 72 | PP_3746 |
| glcF | F6476_01175 | Glycolate oxidase Fe‐S subunit | 100 | 77 | PP_3747 |
| glcG | F6476_01170 | Uncharacterized protein | 90 | 73 | PP_3748 |
| glcB | F6476_01165 | Malate synthase | 99 | 63 | PP_0356 |
| glcA | F6476_01160 | Glycolate permease | 100 | 81 | PP_4735 |
| maeB | F6476_01155 | Malate dehydrogenase/malic enzyme | 98 | 71 | PP_5085 |