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RNP‐directed homologous recombination frequencies using homology arms of various lengths.
| Homology arm size (kb) | No. of analysed transformants | Loss lae1 function | HDR frequency (%) | NHEJ frequency (%) |
|---|---|---|---|---|
| 1 | 21 | 21 (100%) | 100.00 | 0.00 |
| 0.2 | 23 | 23 (100%) | 100.00 | 0.00 |
| 0.05 | 23 | 23 (100%) | 73.91 | 26.09 |
| 0.02 | 23 | 23 (100%) | 56.52 | 43.48 |
