Fig. 8.

Depiction of the PVA pathway, which serves as an intracellular reporter system for the shikimate pathway.

A. Enzymes from the PVA pathway that were overexpressed in Y. lipolytica. (A) Strains expressing the PVA pathway in different genetic backgrounds. The strains’ names are indicated as well as the shikimate pathway overexpressed variant genes and the overexpression of the PVA pathway.

B. Schematic representation of the shikimate pathway and how the flux was modified when genes upstream from the chorismate branching point were overexpressed. This approach can increase the flux to TRP and consequently the production of PVA (strain JMY7795).

C. Schematic representation of the shikimate pathway and how the flux was modified when genes downstream from the chorismate branching point were overexpressed. This approach decreases the TRP that is available for PVA production, resulting in much lighter green cultures (strain JMY7793). The bidirectional arrows indicate reversible reactions.