Fig. 6.
Effect of efflux pump and magnesium ion addition on β‐bisabolene accumulation during cultures on WCO. The cultivation was performed at 200 rpm with an initial OD600 of 0.1 and 10% of n‐dodecane in 50 ml of liquid WCO medium in a 250‐ml shake flask for 5 days. Bars represent bisabolene titers, and lines represent titer fold improvements over controls. All values presented are the mean of three biological replicates ± standard deviation. *P < 0.05, significantly different from control by ANOVA.