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. 2021 Nov 4;11:778331. doi: 10.3389/fcimb.2021.778331

Figure 1.

Figure 1

(A) AB5075 Wild-type opaque (VIR-O) and translucent (AV-T) colonies, viewed under a dissecting microscope with oblique lighting from underneath. (B) Qualitative assay of AHL secretion in which cultures of the wild-type (VIR-O), the VIR-O Δ1132 mutant, the complemented mutant (VIR-O Δ1132/Tn7-1132), and a version of the complemented mutant disrupted by transposon insertion (VIR-O Δ1132/Tn7-1132::T26) were spotted onto a soft agar lawn containing X-Gal and an Agrobacterium tumefaciens traG::lacZ biosensor that reacts to the presence of exogenous AHL by cleaving X-Gal, forming a blue halo. (C) Qualitative assay of AHL secretion in the wild-type (AV-T), the AV-T Δ1132 mutant, the complemented mutant (AV-T Δ1132/Tn7-1132), and a version of the complemented mutant disrupted by transposon insertion (AV-T Δ1132/Tn7-1132::T26). Signal secretion was analyzed as in panel (B), except the amount of X-gal was increased 2-fold. (D) Surface-associated motility of wild-type (VIR-O), the VIR-O Δ1132 mutant, and the complemented mutant (VIR-O Δ1132/Tn7-1132) measured on 0.3% Eiken Agar plates. A Welch’s ANOVA (****p < 0.00005) was carried out to assess (D) error bars indicate standard deviation of the mean.