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Expression and purification of recombinant PRRSV-N protein. (A) SDS-PAGE analysis of the recombinant PRRSV-N protein. (B) Antigenic analysis of the recombinant PRRSV-N protein using the positive pig sera for PRRSV as first antibody. M: Molecular weight markers; Lane 1: pET28a vector control; Lane 2: Induction with 0.1 mM IPTG; Lane 3: Soluble protein in supernatant after sonication; Lane 4: Inclusion body in precipitation after sonication; Lane 5: Purified PRRSV-N protein.
