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. 2021 Nov 18;59(12):e01580-21. doi: 10.1128/JCM.01580-21

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Copyright © 2021 Duan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Establishment of HEK293S cell lines for stable expression of PRRSV-N-Nb1-HRP fusion protein. (A) Detection of the binding between PRRSV-N-Nbs-HRP and PRRSV-N using direct ELISA. (B) Specific reactions between PRRSV-N-Nbs-HRP and PRRSV-N using direct ELISA. (C) Comparisons of the two nanobodies blocking the binding between the pig sera and PRRSV-N protein by cELISA. (D) Characterization of HEK293S cell lines for stably expressing PRRSV-N-Nb1-HRP by fluorescence microscopy. (E) Confirmation of the blocking effect of PRRSV-N-Nb1-HRP using the supernatant from the recombinant HEK293S cell lines by cELISA.