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. 2021 Nov 18;59(12):e01580-21. doi: 10.1128/JCM.01580-21

FIG 5.

FIG 5

Bioactivity analysis of the PRRSV-Nb1-HRP fusion protein from the stably expressing HEK293S cell lines. (A) Expression level of PRRSV-N-Nb1-HRP fusion protein between stable expression in HEK293S and transient transfection in HEK293T cells. (B) Stability of PRRSV-N-Nb1-HRP fusion protein from the two systems. (C) Stability of the PRRSV-N-Nb1-HRP fusion protein from the different generations of stably expressing HEK293S cell lines. (D) Schematic diagram of the production for PRRSV-N-Nb1-HRP fusion proteins using transient transfection in HEK293T cells. (E) Schematic diagram of the production for PRRSV-N-Nb1-HRP fusion proteins using stable expression in HEK293S cells.