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Primers used in this studya
| Primers | Sequence (5′- 3′) | Usage |
|---|---|---|
| CALL001 | GTCCTGGCTGCTCTTCTACAAGG | Overlap-VHH |
| CALL002 | GGTACGTGCTGTTGAACTGTTCC | |
| VHH-FOR | CAGGTGCAGCTGCAGGAGTCTGGGGGAGR | |
| VHH-REV | CTAGTGCGGCCGCTGAGGAGACGGTGACCTGGGT | |
| PRRSV-N-F | CGCGGATCCATGCCAAATAACAACGGCAAGC | pET28a-PRRSV-N |
| PRRSV-N-R | CCCAAGCTTTCATGCTGAGGGTGATGCTGTG | |
| Nb-F | AACTGCAGATGGAGACCGACACC | pCMV-N1-Nbs-HRP |
| Nb-R | ATAAGAATGCGGCCGCTTAGTGGTGATGGTG |
a Restriction sites are underlined.
