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. 2021 Nov 18;59(12):e01580-21. doi: 10.1128/JCM.01580-21

TABLE 3.

Optimized amount of PRRSV-N protein as the coating antigen and dilution of PRRSV-N-Nb1-HRP fusion protein using the direct ELISAa

Nb1-HRP OD450 values after different antigen coating concentration (μg/mL)
8.0 4.0 2.0 1.0 0
1:10 3.339 3.206 3.08 2.421 0.051
1:100 2.915 2.653 1.724 1.021 0.081
1:500 1.235 0.987 0.712 0.48 0.072
1:1000 0.686 0.497 0.342 0.187 0.075

a The optimal amount of PRRSV-N protein and dilution of PRRSV-N-Nb1-HRP were selected when the OD450nm values of the direct ELISA was approximately 1.0.