FIGURE 7.

Hepatic necrosis, apoptosis, and CD3 positive cell infiltration with perfusate sLC or nLC. Hematoxylin and eosin staining (A) and necrosis scores with a circle that is blank as no necrosis, pink as single cell to 30% necrosis, bright red as 30%–60% necrosis, and dark red as >60% necrosis (B); the representative Western blotting band of CCP-3 (C) and densitometry analysis (D); and representative immunofluorescence staining of CD3 cells in the livers (E) and counting (F). P < 0.05 is considered as significant. CD3, cluster of differentiation 3; CCP-3, cleaved caspase-3; nLC, nonsuccessful lactate clearance; NMP, normothermic machine perfusion; sLC, successful lactate clearance.