KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti–mouse CD3 (clone 17A2) | BioLegend | RRID:AB_2715571 |
| anti–mouse CD4 (clone GK1.5) | eBioscience | RRID:AB_469533 |
| anti–mouse CD8 (clone 53–6.7) | eBioscience | RRID:AB_467086 |
| anti–mouse CD45 (clone 30F11) | BioLegend | Catalog 103114 |
| anti–mouse Gr-1 (clone RB6-8C5) | eBioscience | RRID:AB_1210822 |
| anti–mouse CD45 (clone 30-F11) | eBioscience | RRID:AB_469717 |
| anti–mouse CD11b (clone M1/70) | BioLegend | RRID:AB_2715571 |
| anti–mouse CD206 (clone C068C2) | BioLegend | Catalog 141729 |
| anti–mouse Ly6C (clone HK1.4) | BioLegend | Catalog 128004 |
| anti–mouse Ly6G (clone 1A8) | BioLegend | Catalog 127604 |
| anti–mouse CPT1A (clone ab171449) | Abcam | Catalog ab171449 |
| anti–mouse β2-AR (Catalog orb15065) | Biorbyt | Catalog orb15065 |
| anti-human CD33 (clone P67.6) | BioLegend | Catalog 366605 |
| anti-human CD33 (D3HL60.251) | Beckman Coulter | Catalog A54824 |
| anti-human HLA-DR (clone L243) | eBioscience | RRID:AB_1907430 |
| anti-human CD11b (clone D12) | BD Biosciences | Catalog 340937 |
| anti-human CD14 (clone M5E2) | BD Biosciences | RRID:AB_2033939 |
| anti-human CD14 (MϕP9) | BD Biosciences | Catalog 562691 |
| anti-human CD15 (clone HI98) | eBioscience | RRID:AB_464953 |
| anti-human CD4 (clone A161A1) | Biolegend | Catalog 357405 |
| anti-human CD8 (clone RPA-T6) | Biolegend | Catalog 301007 |
| AMPK | Cell signaling technologies | Catalog 5831 |
| phospho-AMPK | Cell signaling technologies | Catalog 2535 |
| ATG5 | Cell signaling technologies | Catalog 2630 |
| COX2 | Cell signaling technologies | Catalog 12282 |
| β-Actin | Cell signaling technologies | Catalog 3700 |
| Chemicals, peptides, and recombinant proteins | ||
| Mouse IL-6 | MilliporeSigma | Catalog I9646 |
| Human IL-6 | MilliporeSigma | Catalog I1395 |
| Mouse GM-CSF | Biolegend | Catalog 713704 |
| Human GM-CSF | Biolegend | Catalog 713604 |
| Mouse GM-CSF | Biolegend | Catalog 713502 |
| Human GM-CSF | Biolegend | Catalog 713402 |
| CellTrace Calcein Violet | ThermoFisher Scientific | Catalog C34858 |
| Propranolol | MilliporeSigma | Catalog P0884 |
| Isoproterenol | MilliporeSigma | Catalog I6504 |
| Etomoxir | Tocris | Catalog 4539 |
| LPS | MilliporeSigma | Catalog L3024 |
| Torin-1 | Cell Signaling | Catalog 14379S |
| 3-MA | Torics | Catalog 3977 |
| Chloroquine | Enzo Life Sciences | Catalog 51031 |
| Critical commercial assays | ||
| CYTO-ID® Autophagy detection kit 2.0 | Enzo Life Sciences | Catalog ENZ-51031-0050 |
| Cell Mito Stress Test Kit | Agilent | Part# 103010-100 |
| Palmitate Oxidation Stress Test Kit | Agilent | Part# 103693-100 |
| Long Chain Fatty Acid Oxidation Stress Test Kit | Agilent | Part# 103672-100 |
| Glycolysis Stress Test Kit | Agilent | Part# 103017-100 |
| Prostaglandin E2 Parameter Assay kit | R & D Systems | Catalog KGE004B |
| Deposited data | ||
| RNA Sequencing | FigShare | https://figshare.com/articles/dataset/RNA_sequencing_data_from_MDSCs_sorted_from_4T1_tumor_bearing_WT_or_beta-2_adrenergic_receptor_knockout_mice_/16691767 |
| Experimental models: cell lines | ||
| 4T1 | ATCC | Catalog CRL-2539 |
| EL4 | ATCC | Catalog TIB-39 |
| Mycoplasma Plus PCR Primer Set | Agilent Technologies | Catalog 302008 |
| Experimental models: organisms/strains | ||
| BALB/c | Charles River | Strain Code: 028 |
| C56BL/6 | Charles River | Strain Code: 027 |
| β2-AR knockout (BALB/c) | David Farrar (UT Southwestern Medical Center) | NA |
| β2-AR knockout (C57BL/6) | RPCCC | NA |
| SCID | RPCCC | NA |
| Software and algorithms | ||
| Prism | GraphPad | https://www.graphpad.com/ |