Skip to main content
. 2021 Nov 18;16(11):e0258312. doi: 10.1371/journal.pone.0258312

Fig 3. LINC01857 competitively binds to miR-197-3p to improve AGR2 expression.

Fig 3

A, LINC01857 expression in HCC cells detected by fractionation of nuclear and cytoplasmic RNA and RT-qPCR. B, fluorescence localization of LINC01857 in HCC cells verified by RNA-FISH. C, binding relation between LINC01857 and miR-197-3p verified by RIP assay; D: binding sites between LINC01857 and miR-197-3p and between miR-197-3p and AGR2 predicted by RNA22 and TargetScan database. E-F, binding relations between LINC01857 and miR-197-3p and between miR-197-3p and AGR2 certified by RIP assay and dual-luciferase reporter gene assay. G, expression of miR-197-3p and AGR2 mRNA in HCC and paracancerous tissues examined by RT-qPCR, n = 54. H, expression of miR-197-3p and AGR2 mRNA in HCC cells assessed by RT-qPCR. Three independent repeated cell tests were conducted. The results were presented as mean ± standard deviation. The paired t-test was employed to analyze the data in panels G/F. One-way ANOVA was used to analyze the data in panel H. Tukey’s multiple comparisons test was applied for post hoc test. Two-way ANOVA was employed to analyze the data in panels A, C, E, and H. Tukey’s multiple comparisons test or Sidak’s multiple comparisons test was employed for the post hoc test. ** p<0.01, *** p<0.001.