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A; Coomassie-stained SDS-PAGE of hPPO variants purified by Ni-NTA affinity chromatography. Position of constructs marked by arrows. B; Substrate conversion rates of E.coli-expressed hPPO variants using protoporphyrinogen IX as a substrate (N.D., not determined). C; Specific activity of hPPO in the presence of various detergents estimated via conversion of protoporphyrinogen IX.
