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. 2021 Nov 18;16(11):e0260206. doi: 10.1371/journal.pone.0260206

Fig 5. Surface expression of PD-L1, PD-1 and ICOS on activated TREGs.

Fig 5

A) Representative flow cytometry dot plots for the proliferation of CTV-stained TEFFs on D4, D5 and D7 of co-culture in the absence or the presence of CFSE-stained TREGs. The TEFF:TREG ratio was 1:0 in the top plot and 1:0.5 in the bottom plot. B) Left panel: representative histograms of receptor expression (solid black lines) on the gated, CFSE-stained TREGs on D4, D5 and D7 of co-culture. Right panel: graphs of each marker’s geometric mean fluorescence intensity at the different co-culture time points for CFSE-stained TREGs in independent experiments (n = 2 to 12, depending on the marker and the time course studied). The isotype control (Iso) is shown for each marker (grey lines). * p≤0.05 in an M-W test. Bars represent the means of all experiments for each proteins. Each black dot represents an experiment, and the open circle represents the same experiment.