Table 3. Range of prostaglandin concentrations reported using different measurement techniques.
| Biofluid | PG/Metabolite | Measurement Technique | Range |
|---|---|---|---|
| plasma | PGE | RIA | NL: 4.8 [36]–3641.2 [69] (pg/ml), L: 5.4 [36]–2429.1 [69] (pg/ml) |
| PGF | RIA | NL: 6.2 [36]–480 [29] (pg/ml), L: 7.9 [36]–600 [29] (pg/ml) | |
| PGE2 | TLC and biological assay | NL: <200 pg/ml [10] | |
| RIA | NL: 4.6 [60]–15,600 [55] (pg/ml), L: 559 [50]–21,200 [55] (pg/ml) | ||
| PGE1 | TLC and biological assay | <200 pg/ml [10] | |
| RIA | NL: 2600 [55]–10,000 [33] (pg/ml), L: 4500 [64]–6800 [33] (pg/ml) | ||
| PGF2α | TLC and biological assay | NL: <200 pg/ml [10], L: <200 [10]– 18,000 [10] (pg/ml) | |
| GC-MS | NL: <70 [19]–600 [19] (pg/ml), L: <100 [19]–200 [19] (pg/ml) | ||
| RIA | NL: 17 [37]–4600 [55] (pg/ml), L: 33.1 [21]–7900 [55] (pg/ml) | ||
| PGF1α | TLC and biological assay | NL: <200 pg/ml [10] | |
| 6-keto-PGF1α | GC-MS | NL: 131 [42]–244 [42] (pg/ml) | |
| RIA | NL: 18.7 [53]–318.6 [58] (pg/ml), L: 21 [70]–608 [70] (pg/ml) | ||
| PGEM | RIA | NL: 58 [54]–554 [77] (pg/ml), L: 82 [54]–433 [77] (pg/ml) | |
| Bicyclo-PGEM | RIA | NL: 49 [48]–200 [59] (pg/ml), L: 62 [48]–500 [75] (pg/ml) | |
| PGFM | GC-MS | NL: 31 pg/ml [19], L: 267–942 [19] (pg/ml) | |
| RIA | NL: 32.1 [61]–490 [41] (pg/ml), L: 20 [75]–2880 [58] (pg/ml) | ||
| serum | PGF2α | RIA | NL: 200 [13]–1800 [12] (pg/ml), L: 100 [11]–3000 [11] (pg/ml) |
| AF | PGE | RIA | NL: 89 [37]–1400 [29] (pg/ml), L: 502.8 [69]–8800 [29] (pg/ml) |
| PGF | RIA | NL: 50 [27]–1650 [24] (pg/ml), L: 500 [27]–75,000 [27] (pg/ml) | |
| PGE2 | GC | NL: <200 [16]–6200 [16] (pg/ml), L: 1200 [16]–17,000 [16] (pg/ml) | |
| PC and TD | NL: 250 [26]–300 [26] (pg/ml), L: 1700 pg/ml [26] | ||
| RIA | NL: <10 [30]–11,177 [80] (pg/ml), L: 17.8 [76]–28,197 [74] (pg/ml) | ||
| ELISA | NL: 24 [85]–4749 [85] (pg/ml), L: 62 [85]–36,651 [85] (pg/ml) | ||
| LC-MS | NL: <10 [87]–70,493 [87] (pg/ml), L: <10 [87]–105,739 [87] (pg/ml) | ||
| PGE1 | GC | NL: <500 pg/ml [16], L: <500 pg/ml [16] | |
| PC and TD | NL: 1000 [26]–1200 [26] (pg/ml), L: 1800 pg/ml [26] | ||
| RIA | NL: <10 [30]–5000 [33] (pg/ml), L: 4400 pg/ml [33] | ||
| PGF2α | RIA | NL: 29 [66]–4700 [26] (pg/ml), L: 27 [71]–44,270 [33] (pg/ml) | |
| ELISA | NL: 8 [85]–926 [85] (pg/ml), L: 78 [85]–15,326 [85] (pg/ml) | ||
| LC-MS | NL: <10 [87]–127 [91] (pg/ml), L: <10 [87]–42,537 [87] (pg/ml) | ||
| PGF1α | PC and TD | NL: 1500 [26]–2000 [26] (pg/ml), L: 12,000 pg/ml [26] | |
| 6-keto-PGF1α | RIA | NL: 67 [79]–809 [80] (pg/ml), L: 68 [80]–1927 [80] (pg/ml) | |
| PGEM | LC-MS | NL: 71 pg/ml [92], L: 8425 pg/ml [92] | |
| Bicyclo-PGEM | RIA | L: 75 [71]- 4275 [71] (pg/ml) | |
| LC-MS | NL: <10 [87]–66,900 [87] (pg/ml), L: 8361 [87]–133,800 [87] (pg/ml) | ||
| 15-keto-PGE2 | LC-MS | NL: 0 pg/ml [92], L: 210.24 pg/ml [92] | |
| 19-OH-PGE2 | LC-MS | NL: 0 [92]–221,100 [87] (pg/ml), L: 73.7 [92]–202,675 [87] (pg/ml) | |
| PGFM | RIA | NL: 80 [79]–1571 [79] (pg/ml), L: 105 [71]–25,028 [56] (pg/ml) | |
| LC-MS | NL: <10 [87]–114.79 [91] (pg/ml), L: <10 [87]–28,360 [87] (pg/ml) | ||
| PGD2 | RIA | L: 900 [63]–1800 [63] (pg/ml) | |
| PGJ2 | LC-MS | L: 8542.8 pg/ml [87] | |
| 9α,11β-PGF2 | ELISA | NL: 30 [84]–204 [84] (pg/ml), L: 10 [84]–396 [84] (pg/ml) | |
| PGA2 | LC-MS | NL: <10 [87]–16,722 [87] (pg/ml), L: <10 [87]–50,167 [87] (pg/ml) | |
| urine | PGF2α | RIA | NL: 0.99 [83]–1.85 [83] (pg/g creatinine), L: 2.03 [83]–3.14 [83] (pg/g creatinine) |
| GC-NICI-MS | NL: 1840 [90]–2060 [89] (pg/ml) | ||
| 6-keto-PGF1α | RIA | NL: 114,000 [67]–571,000 [67] (pg/g creatinine), L: 426,980 [62]–1,219,000 [67] (pg/g creatinine) | |
| PGFM | RIA | NL: 1.82 [83]–4.87 [83] (pg/g creatinine), L: 7.93 [83]–12.70 [83] (pg/g creatinine) | |
| t-PGFM | RIA | NL: 0.46 [20]–2.32 [20] (μg/hr), L: 1.06 [41]–2.50 [31] (μg/hr) | |
| 2,3-dinor-6-keto-PGF1α | ELISA | NL: 623,232 [81]–1,096,181 [81] (pg/ml) |
Published data presented as ng/ml, nanomolars, or picomolars were converted to pg/ml and data presented as ng/g creatinine or ng/mmol creatinine were converted to pg/g creatinine. Data published in μg/hr were not converted and are presented as in the original article.
Abbreviations: RIA = radioimmunoassay, NL = non-labour, L = labour, TLC = thin layer chromatography, GC-MS = gas chromatography-mass spectrometry, PGEM = 13,14-dihydro-15-keto-PGE2, bicyclo-PGEM = 11-deoxy-13,14-dihydro-15-keto-11,16-bicyclo PGE2, PGFM = 13,14-dihydro-15-keto-PGF2α, PC = paper chromatography, TD = transmission densitometry, ELISA = enzyme-linked immunosorbent assay, LC-MS = liquid chromatography-mass spectrometry, GC-NICI-MS = gas chromatography-negative ion chemical ionization-mass spectrometry, t-PGFM = 5α,7α-dihydroxy 11-keto tetranor-prostane 1,16-dioic acid.