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. Author manuscript; available in PMC: 2023 Nov 1.
Published in final edited form as: Mass Spectrom Rev. 2021 Feb 10;41(6):901–921. doi: 10.1002/mas.21688

Figure 3.

Figure 3.

General workflows for Released N-linked glycan quantitation by MALDI-TOF MS (A) and by LC-ESI-MS (B). In MALDI-TOF MS analysis, labeled N-linked glycans are mixed with a MALDI-matrix and irradiated with laser shots to collect MS data (A). In LC-ESI-MS, labeled or unlabeled N-linked glycans are separated by using a liquid chromatography method, followed by ESI-MS analysis (B). During LC-MS analysis of labeled glycans, the label is most commonly installed at the reducing end. By contrast, a common labeling strategy for MALDI-MS involves derivatizing sialic acids, which are at the nonreducing end.