Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | AtmR35X, AtmTm1.1(103CAG)TGAMfgc | This paper | 103C>T mutation, human exon replacement |
Generated by Hicks laboratory. Has been backcrossed into C57b/6 9 times. Contact pmathews@lundquist.org |
| Strain, strain background (M. musculus) | AtmQ35X, AtmTm1.1(103C)TMfgc | This paper | 103C>T mutation, targeted premature termination signal in the mouse codon |
Generated by Hicks laboratory. Has been backcrossed into C57b/6 9 times. Contact pmathews@lundquist.org |
| Strain, strain background (M. musculus) | Aptx−/− | Ahel et al., 2006 | MGI Cat# 3687171, RRID:MGI:3687171 | Contact peter.mckinnon@stjude.org |
| Gene (M. musculus) | Atm | MGI | MGI:107202; C030026E19Rik; ENSMUSG00000034218 | |
| Gene (Homo Sapien) | ATM | OMIM | OMIM: 607585 MGI: 107202 HomoloGene: 30952; ENSG00000149311 | |
| Sequence-based reagent | Atm gene | Transnetyx | PCR primers | F-5′-CCTTTGAGGCATAAGTTGCAACTTG-3′ |
| Sequence-based reagent | Atm gene | Transnetyx | PCR primers | R-5′-GTACAGTGTATCAGGTTAGGCATGC-3′ |
| Chemical compound/ drugs | GJ103 saltFormula: C16H14N403S | TargetMol | T3448;CAS No.: 1459687-96-7 | 100 μM in media |
| Antibody | Anti-mouse CD68 (Rat monoclonal) | Bio-Rad | Cat# MCA1957, RRID: AB_322219 |
IF (1:400) |
| Antibody | ATM (D2E2) (Rabbit- monoclonal) | Cell Signaling Technology | Cell Signaling Technology Cat# 2873, RRID:AB_2062659 | WB (1:500)WB (1:1000) |
| Antibody | GAPDH (14C10)(Rabbit-monoclonal) | Cell Signaling Technology | Cell Signaling Technology Cat# 2118, RRID:AB_561053 | WB (1:4000) |
| Antibody | β-Actin (D6A8)(Rabbit-monoclonal) | Cell Signaling Technology | Cell Signaling Technology Cat# 8457, RRID:AB_10950489 | WB (1:5000) |
| Antibody | Anti-Rabbit IgG, HRP-linked(Goat-monoclonal-polyclonal) | Cell Signaling Technology | Cell Signaling Technology Cat# 7074, RRID:AB_2099233 | WB (1:5000) |
| Antibody | Anti-Calbindin (D-28k)(Rabbit, polyclonal) | Swant Inc. | Swant Cat# CB 38, RRID:AB_10000340 | IF (1:1000) |
| Antibody | Anti-Mouse Alexa Fluor 488 (Goat polyclonal) | Thermo Fisher Scientific; Invitrogen | Cat# A11001, RRID: AB_2534069 |
IF (1:500) |
| Antibody | Anti-mouse Cleaved Caspase-3, Asp-175 (rabbit) | Cell Signaling Technology | Cat# 9961, RRID: AB_2341188 |
IF (1:200) |
| Antibody | Anti-Rat Alexa Fluor 555 (Goat polyclonal) | Thermo Fisher Scientific; Invitrogen | Cat# A21244, RRID: AB_2535812 |
IF (1:1000) |
| Antibody | Anti-Rabbit Alexa Fluor 647 (Goat polyclonal) | Thermo Fisher Scientific; Invitrogen | Cat# A-21434, RRID: AB_141733 |
IF (1:500) |
| Antibody | Anti-Calbindin D-28k (mouse-monoclonal) | Swant Inc. | Cat# CB300 | IF (1:500) |
| Antibody | Anti-Rabbit Alexa Fluor 488 (Goat-polyclonal) | Thermo Fisher Scientific;Invitrogen | Thermo Fisher Scientific Cat# A-11034, RRID:AB_2576217 |
IF (1:1000) |
| Antibody | CD4(GK1.5)(Rat-monoclonal) | Thermo Fisher Scientific;Invitrogen | Thermo Fisher Scientific Cat# 50-0041-82, RRID:AB_10609337 |
FACS(5 µl per test) |
| Antibody | CD8(53-6.7)(Rat-monoclonal) | Thermo Fisher Scientific;Invitrogen | Thermo Fisher Scientific Cat# 53-0081-82, RRID:AB_469897 |
FACS(5 µl per test) |
| Antibody | CD3(145-2C11)(Hamster-monoclonal) | Thermo Fisher Scientific;Invitrogen | Thermo Fisher Scientific Cat# 12-0031-83, RRID:AB_465497 |
FACS(5 µl per test) |
| Antibody | CD44(IM7)(Rat-monoclonal) | Thermo Fisher Scientific;Invitrogen | Thermo Fisher Scientific Cat# 25-0441-82, RRID:AB_469623 |
FACS(5 µl per test) |
| Antibody | CD25(PC61.5)(Rat-monoclonal) | Thermo Fisher Scientific;Invitrogen | Thermo Fisher Scientific Cat# 47-0251-82, RRID:AB_1272179 |
FACS(5 µl per test) |
| Other | Eosin Y (Certified Biological Stain) | Thermo Fisher Scientific (Fisher Chemical) | Cat# E511-100 | |
| Other | Hematoxylin Stain Solution, Modified Harris Formulation, Mercury Free Nuclear Stain | RICCA Chemical Company | Cat# 3530-16 | |
| Other | Permount Mounting Medium | Thermo Fisher Scientific (Fisher Chemical) | Cat# SP15-100 | |
| Other | Fluoromount-G with DAPI | Southern Biotech | Cat# 0100-20, RRID: SCR_021261 |
|
| Commercial assay or kit | BCA Protein Assay Kit | Thermo Fisher Scientific;Pierce | Cat# 23225 | Protein assay |
| Commercial assay or kit | SuperSignal West Pico Chemiluminescent Substrate | Thermo Fisher Scientific;Pierce | Cat# 34580 | Chemiluminescent substrate |
| Commercial assay or kit | Radiance plus | Azure Biosystems | Cat# AC2103 | Chemiluminescent substrate |
| Software, algorithm | FlowJo | https://www.flowjo.com/solutions/flowjo | RRID:SCR_008520 | |
| Software, algorithm | ImageJ software | ImageJ (http://imagej.nih.gov/ij/) | RRID:SCR_003070 | Version 1.53 |
| Software, algorithm | IgorPro | http://www.wavemetrics.com/products/igorpro/igorpro.htm | RRID:SCR_000325 | Version 7; Tarotools procedures |
| Software, algorithm | Neuroexpress | https://www.researchgate.net/project/NeuroExpress-Analysis-software-for-whole-cell-electrophysiological-data | https://www.researchgate.net/project/NeuroExpress-Analysis-software-for-whole-cell-electrophysiological-data | Version 21.1.13; used for sEPSC analyses |
| Software, algorithm | GraphPad, Prism | GraphPad Prism (https://graphpad.com) | RRID:SCR_015807 | Versions 8 and 9 |
| Software, algorithm | MBF, Stereo investigator | https://www.mbfbioscience.com/stereology | RRID:SCR_017667 | Version 2021 |
| Software, algorithm | Microsoft Excel | https://www.microsoft.com/en-us/microsoft-365/excel | RRID:SCR_016137 | Version 365 |
| Software, algorithm | Catwalk XT | https://www.noldus.com/catwalk-xt | RRID: SCR_021262 |