Fig. 2.

Identification of DEGs in isl2b:GFP⁺ RGCs after ONT. (A) Experimental workflow for FACS-isolation of isl2b:GFP⁺ RGCs. An example FACS plot showing a cell-sorting gate is included. Icons were adapted from BioRender.com. Number of experiments=1. (B) Heatmap showing hierarchical clustering of 308 DEGs at 24 hpi from three biological replicates. (C) Heatmap highlighting DEGs of interest based on known neuroprotective and pro-regenerative functions. Heatmap legends show log₂TPM. (D) Pathway enrichment analysis using the KEGG database showing top-3 down- and upregulated pathways after ONT.