TABLE 6.

Fermentative performances at 30°C of Saccharomyces cerevisiae strain Fm17 and the benchmark S. cerevisiae Ethanol Red (ER) when incubated in the presence of pre-hydrolysate Cc3 and Pa3 supplemented with 20, 40, or 92 g/L glucose.

Glucose concentration (g/L)	Pre-hydrolysate	Highest glycerol concentration (g/L)		Highest ethanol concentration (g/L)		YE/G (g/g)		Q (g/L h–1)		Qmax (g/L h–1)
		Fm17	ER	Fm17	ER	Fm17	ER	Fm17	ER	Fm17	ER
20	-	0.79 ± 0.03	0.74 ± 0.03	8.17 ± 0.38	8.21 ± 0.38	0.41 (80%)	0.41 (80%)	0.31	0.32	0.40	0.40
20	Cc3	0.83 ± 0.03	0.74 ± 0.04	9.34 ± 0.27	8.26 ± 0.21	0.46 (90%)	0.40 (79%)	0.36	0.41	0.47	0.41
20	Pa3	0.80 ± 0.04	0.72 ± 0.03	9.04 ± 0.28	8.01 ± 0.25	0.45 (88%)	0.40 (78%)	0.45	0.31	0.45	0.39
40	-	1.51 ± 0.06	1.64 ± 0.07	16.92 ± 0.77	16.95 ± 0.80	0.42 (82%)	0.42 (82%)	0.38	0.39	0.82	0.84
40	Cc3	1.65 ± 0.07	1.55 ± 0.07	18.29 ± 0.43	17.25 ± 0.40	0.45 (88%)	0.42 (82%)	0.66	0.70	0.90	0.85
92	-	3.27 ± 0.13	3.27 ± 0.15	35.56 ± 1.54	36.34 ± 1.39	0.40 (79%)	0.41 (80%)	0.81	0.83	1.33	1.35
92	Pa3	3.80 ± 0.17	3.63 ± 0.16	39.09 ± 1.42	37.60 ± 1.00	0.45 (88%)	0.43 (85%)	0.86	0.85	1.31	1.29

The same glucose concentrations were supplemented to YNB as reference broth. The highest glycerol and ethanol levels were reported. All experiments were conducted in triplicate (± SD). Y_E/G, ethanol yield per gram of consumed glucose calculated on the highest ethanol production and % of theoretical maximum indicated in brackets; Q, volumetric productivity at the highest ethanol production.