Fig. 5.

XFBD inhibits inflammation-induced macrophage recruitment in zebrafish. (a) Illustration of the timeline of drug protection and tail amputation. Red dotted line represents the amputation site, and the blue box represents the region for the cell counting of recruited inflammatory cells. (b) Toxicity effects of different doses of XFBD (50–800 μg·mL⁻¹) on fish survival and general development. (c) Representative images and (d) quantification of the accumulation of macrophages at the wounding sites of the control or the XFBD-treated (50–200 μg·mL⁻¹) embryos. (e) Representative images and (f) quantification of the accumulation of neutrophils at the wounding sites of the control or the XFBD-treated (50–200 μg·mL⁻¹) embryos. (g) Time-lapse imaging of macrophage movement in the control and the XFBD-treated (200 μg·mL⁻¹) embryos at indicated times after tail amputation. (h) Quantification of the moving velocity of macrophages. (i) Gene expression of cytokines IL-6 and IL-1β in tail-amputated embryos with or without XFBD (200 μg·mL⁻¹) treatment. In (c, e, g), white dotted lines outline the fish embryos; yellow (c, e) or red (g) dashed lines mark the transection site; yellow and red asterisks (g) mark the moving trajectory of the representative macrophages. mpa: minutes post amputation; UC: tail-uncutted embryos; CTR: tail-cutted embryos without treatment; XFBD group: tail-cutted embryos with XFBD treatment of different dosages. *: P < 0.05; **: P < 0.01.