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Analytical fractionation by gel filtration on a HiLoad 16/60 Superdex 200 column of a crude Zymolyase extract of a C. albicans ATCC 66369 germ tube. The crude extract was applied to the column and was then eluted with PBS (pH 7.2). The optical density at 280 nm (⧫) was monitored during elution, and 10 μl of each fraction was analyzed by ELISA with MAb 16B1-F10 (×) as the probe.
