Physical and microbial characteristics of larval sites in La Lopé (a, c, e) and Rabai (b, d, f). (a‐b) Principal component analysis (PCA) of all physical variables of larval sites. The first two PCs are shown, and the variance explained by each PC is indicated in the axis label. Each point represents a single larval site. Colors of points indicate habitat, and shapes of points indicate whether Ae. aegypti were found in the sites (i.e., Ae. aegypti presence status). In the legend, “+” and “−” denote Ae. aegypti present and absent sites, respectively. An ellipse is drawn for each larval site group with a 75% confidence level. The colors of ellipses represent habitat types and match the colors of points. The solid and dashed ellipses correspond to Ae. aegypti present and absent sites. The original variables are overlaid as vectors on the PC1‐PC2 plate with major variables labeled. The vectors indicate the linear coefficients of the original variables (i.e., the loadings) on the first two PCs. (c‐d) Comparison of microbial densities of larval sites. The boxplots show the minimum, 25% quartile, median, 75% quartile, and maximum. Asterisks indicate statistically significant differences (p < .05). (e‐f) Non‐metric multidimensional scaling (NMDS) analysis of bacterial community compositions. The colors and shapes of points and ellipses are the same as in the PCA (panel a‐b)