Table 3.
Cell cytotoxicity assay with Yashtimadhu extract treatment for 48 h.
Sample | Replicate | 560 nm | 630 nm | Background subtraction | Blank subtraction | % cell viability | Average % cell viability |
---|---|---|---|---|---|---|---|
Blank | Rep_1 | 0.046 | 0.0325 | 0.0135 | |||
Rep_2 | 0.044 | 0.031 | 0.013 | 0 | NA | NA | |
Rep_3 | 0.046 | 0.0335 | 0.0125 | ||||
Control | Rep_1 | 0.736 | 0.1225 | 0.6135 | 0.6 | 100.00 | 100.00 |
Rep_2 | 0.7575 | 0.1235 | 0.634 | 0.621 | 100.00 | ||
Rep_3 | 0.605 | 0.1005 | 0.5045 | 0.492 | 100.00 | ||
50 µg/ml | Rep_1 | 0.737 | 0.1275 | 0.6095 | 0.596 | 99.33 | 98.13 |
Rep_2 | 0.7775 | 0.13 | 0.6475 | 0.6345 | 102.17 | ||
Rep_3 | 0.592 | 0.1225 | 0.4695 | 0.457 | 92.89 | ||
100 µg/ml | Rep_1 | 0.762 | 0.1175 | 0.6445 | 0.631 | 105.17 | 98.65 |
Rep_2 | 0.807 | 0.145 | 0.662 | 0.649 | 104.51 | ||
Rep_3 | 0.5295 | 0.0925 | 0.437 | 0.4245 | 86.28 | ||
200 µg/ml | Rep_1 | 0.665 | 0.1155 | 0.5495 | 0.536 | 89.33 | 99.18 |
Rep_2 | 0.82 | 0.149 | 0.671 | 0.658 | 105.96 | ||
Rep_3 | 0.622 | 0.1065 | 0.5155 | 0.503 | 102.24 | ||
500 µg/ml | Rep_1 | 0.7235 | 0.152 | 0.5715 | 0.558 | 93.00 | 98.76 |
Rep_2 | 0.7655 | 0.1565 | 0.609 | 0.596 | 95.97 | ||
Rep_3 | 0.6855 | 0.145 | 0.5405 | 0.528 | 107.32 | ||
1000 µg/ml | Rep_1 | 0.598 | 0.1225 | 0.4755 | 0.462 | 77.00 | 78.98 |
Rep_2 | 0.635 | 0.1235 | 0.5115 | 0.4985 | 80.27 | ||
Rep_3 | 0.514 | 0.1095 | 0.4045 | 0.392 | 79.67 | ||
1500 µg/ml | Rep_1 | 0.722 | 0.1435 | 0.5785 | 0.565 | 94.17 | 84.31 |
Rep_2 | 0.56 | 0.1145 | 0.4455 | 0.4325 | 69.65 | ||
Rep_3 | 0.549 | 0.098 | 0.451 | 0.4385 | 89.13 |