TABLE 3.

Primer sequences for quantitative real-time polymerase chain reaction.

Gene	Forward primer (5′)	Reverse primer (3′)
Β-actin	AAG​CCA​CCC​CAC​TTC​TCT​CTA​A	AAT​GCT​ATC​ACC​TCC​CCT​GTG​T
B2M	TGC​TGT​CTC​CAT​GTT​TGA​TGT​ATC​T	TCT​CTG​CTC​CCC​ACC​TCT​AAG​T
YWHAZ	TCT​GTC​TTG​TCA​CCA​ACC​ATT​CTT	TCATGCGGCCTTTTTCCA
ACAN	AGG​GCG​AGT​GGA​ATG​ATG​TT	GGT​GGC​TGT​GCC​CTT​TTT​AC
COL1A2	GCT​ACC​CAA​CTT​GCC​TTC​ATG	GCA​GTG​GTA​GGT​GAT​GTT​CTG​AGA
COL2A1	CTG​CAA​AAT​AAA​ATC​TCG​GTG​TTC​T	GGG​CAT​TTG​ACT​CAC​ACC​AGT
SOX9	CTT​TGG​TTT​GTG​TTC​GTG​TTT​TG	AGA​GAA​AGA​AAA​AGG​GAA​AGG​TAA​GTT​T
COL10A1	GAA​GTT​ATA​ATT​TAC​ACT​GAG​GGT​TTC​AAA	GAG​GCA​CAG​CTT​AAA​AGT​TTT​AAA​CA
RUNX2	GGA​GTG​GAC​GAG​GCA​AGA​GTT​T	AGC​TTC​TGT​CTG​TGC​CTT​CTG​G
VCAN	TGC​TAA​AGG​CTG​CGA​ATG​G	AAA​AAG​GAA​TGC​AGC​AAA​GAA​GA
MMP13	AAA​AAG​GAA​TGC​AGC​AAA​GAA​GA	CGG​AGA​CTG​GTA​ATG​GCA​TCA
ALPL	GCT​GTA​AGG​ACA​TCG​CCT​ACC​A	CCT​GGC​TTT​CTC​GTC​ACT​CTC​A