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. 2021 Nov 18;6:75. doi: 10.1038/s41536-021-00185-5

Fig. 2. cECFC administration promotes vessel density and reduces BBB-permeability.

Fig. 2

A Representative immunofluorescent labelling of vessel basement membrane (Col IV; magenta) in the parietal cortex. FGR displayed truncated vasculature with limited branching compared with other groups. B Analysis found a significant decrease in coverage of Col IV-positive vessels which improved following cECFC administration. D Vascular endothelial progenitor cell marker (CD34; red) showed similar patterns observed as Col IV, with FGR demonstrating lower labelled area (C). FGR displayed decreased labelling of the endothelial cell marker CD31, which was higher in cECFC treated brains (E and F). G Analysis of the vasculature showed a significant decrease in vessel length as assessed by labelling of Col IV. H Vessel branching was also reduced in FGR relative to NG. FGR + cECFC did not display increased vascular branching. All values are expressed as mean ± SEM (minimum n = 6 for all groups). Two-way ANOVA with Tukey post-hoc test (*p < 0.05, **p < 0.01) (Scale bars: low magnification: 200 µm; high magnification: 50 µm).