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. 2021 Nov 18;12:6706. doi: 10.1038/s41467-021-26986-2

Fig. 1. Planarian Hox genes are required for adult asexual reproduction.

Fig. 1

a Diagram of the patterning and behavioral aspects of planarian asexual reproduction. b Fission activity following RNAi against Hox genes. Plot depicts fission progeny number on day 12 of primary RNAi screen (n = 54, 12, and 18 animals for Control#1, Control#2, and all other RNAi samples, respectively; p-value calculated by Welch’s two-tailed t-test versus corresponding control; Control#1 vs. lox5a, post2d, hox4b, post2c, lox4, hox4a, post2a, hox3b, hox3a, hox1, lox5b, post2b p-values = 0.1733, 0.5958, >0.9999, 0.8582, 0.4191, 0.0455, 0.0348, <0.0001, <0.0001, <0.0001, <0.0001, and <0.0001, respectively, Control#2 vs. post1 p-value = 0.0384). Box and whisker plot depicts individual data points, median (center), 25th/75th percentile (bounds of box), and minima/maxima (whiskers). Source data are provided as a Source Data file. c, d Heatmaps depicting fission activity following RNAi treatment for both the (c) 2-phase primary and (d) secondary Hox RNAi screens. Cumulative fissions over time are displayed for individual worms from each RNAi condition (n = 12 or 18 animals). Source data are provided as a Source Data file. e, f Representative images of animals from secondary Hox RNAi screen on days 0 and 12 of the fission assay (n = 12 animals, three independent repeats). Animals were given nine dsRNA feedings for primary phase I and 12 dsRNA feedings for phase II screening. Animals were given 17 bacterial RNAi feedings for secondary screening. g Representative images and phenotypic frequency of RNAi animals 15 days post-amputation after eight dsRNA feedings (n = 18–53 animals, three independent repeats). Scale, 1 mm.