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. 2021 Nov 18;7:81. doi: 10.1038/s41522-021-00254-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Top panels: unweighted (a) and weighted (b) UniFrac distance of all samples according to the collection method. Left panels: median (and IQR) distances in within-sample comparisons: preservative samples only (P), non-preservative samples (No P), and across sample sample collection methods—preservative vs non-preservative samples (P/No P). Right panels: PCoA plots of all samples by the sample collection method. Bottom panels: unweighted (c) and weighted (d) UniFrac distances of the paired specimens from six subjects sampled on two consecutive days and according to collection method. Left panels: distance between unrelated subjects (gray) or within an individual across days (pink) separated by collection method: preservative samples (P) and non-preservative samples (no P). Right panels: PcoA plot of all samples with only six multi-day subjects visualized. For all left panels, pairwise Wilcox test with FDR correction **q < 0.01, ***q < 0.001. Lines connect specimens collected from the same subject on the same day (solid) or different days (dotted).