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. 2021 Nov 18;12:6682. doi: 10.1038/s41467-021-26968-4

Fig. 3. Adrenalectomy (ADX) dissipates the anti-depressive effects of RS5 in CRST mice.

Fig. 3

a Experimental design. Mice were exposed to CRST, followed by ADX or sham surgery, and RS5 treatment, and then were placed in the behavioral tests. Red arrows (↓) in Exp #1, Serum collection points. Mice were sacrificed after exposure to a single 5-min restraint (S5). b–h The basal CORT levels at −5 min, and the time course of serum CORT levels in the indicated groups after exposure to S5 (Exp #1) (b) (n = 7–10 animals per group). The % time of social interaction in the SIT (c), the % sucrose-preference in the SPT (d), and immobility time in the TST (e) and FST (f) for the indicated groups (Exp #2). K-means clustering of individuals in the SIT × SPT × [TST × FST] matrix (g) and % composition of each group in the clusters (h). The TST and FST components were transformed into linear eigenvectors using PCA (PVE; 78.2%) (n = 7–10 animals per group). Data were mean ± SEM. Gray circles represent individual data points. *, the difference compared to control; #, the difference compared to CRST; +, the difference compared to CRST + RS5. *, #, +, p < 0.05; **, ##, ++, p < 0.01 (One-way ANOVA followed by Newman–Keuls post hoc test). See Supplementary Data 4 for statistical details.