Figure 2.

Characteristics of the peripheral blood leukocyte clusters indicate the maternal immune inflammatory status in RM patients

A. Heatmap of the top 5 genes expressed in peripheral blood T cell subsets. Subject group and cell type information is indicated above the plot. B. Box plots depicting proportion of various peripheral T cell subtypes in Ctrl and RM patients. C. Violin plots showing the differential expression (absolute log₂ FC > 0.5 and Bonferroni-adjusted P < 0.05) of typical marker genes of MAIT subset in Ctrl and RM patients using the method of non-parametric two-sided Wilcoxon rank sum test in Seurat. D. UMAP visualization of the log-transformed, normalized IFNG expression in CD8T effector subset in Ctrl (left) and RM (right) patients. High expression is shown in red, and low expression in gray. E. Box plots displaying proportion of peripheral NK cell subsets in Ctrl and RM patients. F. Violin plots showing the differential expression (absolute log₂ FC > 0.5 and Bonferroni-adjusted P < 0.05) of anti-inflammation genes including TIGIT, KLRG1, IL32, and ALOX5AP, and pro-inflammation genes including HLA-B, NR4A2, JUN, JUNB, NFKBIA, and MAP3K8 in peripheral NK dim subset from Ctrl and RM patients, using method of the non-parametric two-sided Wilcoxon rank sum test in Seurat. FC, fold change.