Figure 7.

Alterations of dNK subsets in the decidua of RM pregnancy

A. Representative flow cytometry plots showing the strategy to sort dNK cells in human decidual tissues at gestational weeks 6–8, including dNK1 (CD3⁻CD56⁺CD39⁺), dNK3 (CD3⁻CD56⁺CD39⁻CD103⁺), and dNK4 (CD3⁻CD56⁺CD16⁺). B. Quantification of cell population of dNK1 and dNK3 (left panel), as well as dNK4 (right panel) subsets in decidual tissues from Ctrl (n = 5) and RM patients (n = 5 in left panel, and n = 11 in right panel). Significant difference between the two groups is analyzed using unpaired t-test. *, P < 0.05; **, P < 0.01. C. Violin plots illustrating the expression of typical differential genes (absolute log₂ FC > 0.5 and Bonferroni-adjusted P < 0.05) in dNK1, dNK3, and dNK4 cells from Ctrl and RM patients. The differential expression analysis is performed using the method of non-parametric two-sided Wilcoxon rank sum test in Seurat.