Supplementary Figure S1.

Gating strategy and quality control for droplet scRNA-seq A. Flow sorting of living leukocytes from peripheral blood and decidual tissue using CD45 antibody and 7-AAD. B. Total number of cells that met the requirement of quality control and subjected to droplet scRNA-seq. C. Workflow depicting the dissociation and sorting of CD45+ leukocytes from peripheral blood and decidual to generate scRNA transcriptome profile. D. Quality evaluation on sequencing data including the total number of reads per cell (left panel) and total number of genes per cell (right panel) and in peripheral blood and decidua. 7-AAD, 7-aminoactinomycin D.