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. 2021 Aug 24;43(6):2635–2652. doi: 10.1007/s11357-021-00423-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — STEM effects on C2C12 myotube fusion, size, and myotube transcriptional signature: panels represent A quantification of controls (2% and 4% horse serum) and 2–24% STEM-treated C2C12 myotubes for myonuclear fusion (%) and myotube size. Representative images for horse serum and STEM for myonuclear fusion and myotube percent area. B Volcano plot and table of top significantly increased and decreased genes in STEM-treated myotubes compared to horse serum (control). SF denotes serum free media. Results are mean with standard error of the mean, one-way ANOVA. * = different from groups not under solid line