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. 2021 Nov 5;12:757909. doi: 10.3389/fimmu.2021.757909

Figure 2.

Figure 2

Cytosolic hyper-replicating S. Infantis inhibited apoptosis by intermittently phosphorylating Akt. (A) Western blot analysis of p-Akt (Ser473) and p-Akt (Thr308) protein expression levels at 0, 0.5, 1, 2, 3, 4, 5, 6, 7, 8 hpi, respectively. CN, control. (B) Immunoblotting verified the inhibitory effect of MK2206 on p-Akt (Ser473) and p-Akt (Thr308) protein expression levels at 0.5, 3, and 4 hpi. (C) Western blot analysis of Cleaved-caspase-3 and Cleaved-PARP protein expression levels at 0.5, 3, and 4 hpi after after p-Akt was suppressed by MK2206. (D–F) Immunofluorescence staining analysis p-Akt (Thr308) distribution. CN, control; SI, S. Infantis. (D) Distribution of p-Akt (Thr308) in all infected cells at 0.5 hpi. (E) Expression of p-Akt (Thr308) in infected cells containing cytosolic hyper-replicating S. Infantis at 4 and 8 hpi. (F) Expression of p-Akt (Thr308) in infected cells without cytosolic hyper-replicating S. Infantis at 4 and 8 hpi. At least 100 cells were counted for each group. Red: S. Infantis. Green: p-Akt (Thr308). Blue: Hoechst 333342. Data were presented as the mean ± SEM from three independent experiments (n = 3).