Table 1.
Reagents and antibodies.
| Reagents and antibodies | Catalog number | Company/Brand | Origin |
|---|---|---|---|
| Mito-Tracker Red CMXRos | C1035 | Beyotime Biotechnology | Shanghai, China |
| Hoechst 33342 | C1025 | Beyotime Biotechnology | Shanghai, China |
| SC79 | SF2730 | Beyotime Biotechnology | Shanghai, China |
| MK2206 | SF2712 | Beyotime Biotechnology | Shanghai, China |
| Cell Mitochondria Isolation Kit | C3601 | Beyotime Biotechnology | Shanghai, China |
| Enhanced Cell Counting Kit-8 | C0042 | Beyotime Biotechnology | Shanghai, China |
| Alexa Fluor 488-labeled Goat Anti-Rabbit lgG(H+L) | A0423 | Beyotime Biotechnology | Shanghai, China |
| 4’,6’-diamidino-2-phenylindole (DAPI) solution | C0060 | Beijing Solarbio Science & Technology Co., Ltd. | Beijing, China |
| Calcein-AM/PI | CA1630 | Beijing Solarbio Science & Technology Co., Ltd. | Beijing, China |
| Gentamycin Sulfate | G8170 | Beijing Solarbio Science & Technology Co., Ltd. | Beijing, China |
| Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) | C6700 | Beijing Solarbio Science & Technology Co., Ltd. | Beijing, China |
| Chlorquine diphosphate salt | C6628 | Sigma-Aldrich | St. Louis, USA |
| Triton X-100 | T8787 | Sigma-Aldrich | St. Louis, USA |
| Dulbecco’s Modified Eagle Medium (DMEM)/High Glucose | SH30022.01 | GE Healthcare Life Sciences HyClone Laboratories | Utah, USA |
| Phosphate buffered saline (PBS) | SH30256.01 | GE Healthcare Life Sciences HyClone Laboratories | Utah, USA |
| Fetal bovine serum (FBS) | 10099141 | Thermo Fisher Scientific | Rockford, USA |
| Annexin V-PE/7-AAD Apoptosis Detection Kit | A213 | Vazyme Biotech Co., Ltd | Jiangsu, China |
| TUNEL FITC Apoptosis Detection Kit | A111-01 | Vazyme Biotech Co., Ltd | Jiangsu, China |
| Rabbit Anti-LAMP 1 polyclonal antibody | 21997-1-AP | Proteintech Group Inc | Rosemont, USA |
| Rabbit Anti-Occludin polyclonal antibody | 27260-1-AP | Proteintech Group Inc | Rosemont, USA |
| Rabbit Anti-Claudin-1 polyclonal antibody | 13050-1-AP | Proteintech Group Inc | Rosemont, USA |
| Rabbit Anti-Caspase 9/p35/p10 polyclonal antibody | 10380-1-AP | Proteintech Group Inc | Rosemont, USA |
| Rabbit Anti-Bax polyclonal antibody | 50599-2-Ig | Proteintech Group Inc | Rosemont, USA |
| Rabbit Anti-Bcl-2 polyclonal antibody | 12789-1-AP | Proteintech Group Inc | Rosemont, USA |
| Rabbit Anti-VDAC1 polyclonal antibody | 55259-1-AP | Proteintech Group Inc | Rosemont, USA |
| Rabbit Anti-Phospho-Caspase-9 (Ser196) polyclonal antibody | 28794-1-AP | Proteintech Group Inc | Rosemont, USA |
| Rabbit Anti-Cytochrome c monoclonal antibody | 66264-1-Ig | Proteintech Group Inc | Rosemont, USA |
| Mouse Anti-Beta ACTIN monoclonal antibody | 60008-1-lg | Proteintech Group Inc | Rosemont, USA |
| HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) | SA00001-2 | Proteintech Group Inc | Rosemont, USA |
| HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) | SA00001-1 | Proteintech Group Inc | Rosemont, USA |
| Rabbit Anti-Cleaved Caspase-3 monoclonal antibody | 9661T | Cell Signaling Technology | Danvers, USA |
| Mouse Anti-Cleaved PARP monoclonal antibody | 9548T | Cell Signaling Technology | Danvers, USA |
| Rabbit Anti-Phospho-Akt (Ser473) monoclonal antibody | 4060T | Cell Signaling Technology | Danvers, USA |
| Rabbit Anti-Phospho-Akt (Thr308) monoclonal antibody | 13038T | Cell Signaling Technology | Danvers, USA |
| Rabbit Anti- Caspase-1 monoclonal antibody | 24232S | Cell Signaling Technology | Danvers, USA |
| Rabbit Anti-ZO-1 polyclonal antibody | 40-2300 | Thermo Fisher Scientific | Rockford, USA |
| Rabbit Anti-Phospho-Bad (Ser136) polyclonal antibody | ab15098 | Abcam | Cambridge, UK |
| Rabbit Anti GSDMD-N monoclonal antibody | ab215203 | Abcam | Cambridge, UK |