Table 2.
The dilution ratio of primary antibodies.
| Antibody | Dilution rate |
|---|---|
| Rabbit Anti-LAMP 1 polyclonal antibody | 1:2,000 |
| Rabbit Anti-Occludin polyclonal antibody | 1:1,500 |
| Rabbit Anti-Claudin-1 polyclonal antibody | 1:2,000 |
| Rabbit Anti-Caspase 9/p35/p10 polyclonal antibody | 1:500 |
| Rabbit Anti-Bax polyclonal antibody | 1:5,000 |
| Rabbit Anti-Bcl-2 polyclonal antibody | 1:1,000 |
| Rabbit Anti-VDAC1 polyclonal antibody | 1:1,000 |
| Rabbit Anti-Phospho-Caspase-9 (Ser196) polyclonal antibody | 1:1,000 |
| Rabbit Anti-Cytochrome c monoclonal antibody | 1:5,000 |
| Mouse Anti-Beta ACTIN monoclonal antibody | 1:5,000 |
| Rabbit Anti-Cleaved Caspase-3 monoclonal antibody | 1:1,000 |
| Mouse Anti-Cleaved PARP monoclonal antibody | 1:1,000 |
| Rabbit Anti-Phospho-Akt (Ser473) monoclonal antibody | 1:2,000 |
| Rabbit Anti-Phospho-Akt (Thr308) monoclonal antibody | 1:2,000 |
| Rabbit Anti- Caspase-1 monoclonal antibody | 1:1,000 |
| Rabbit Anti-ZO-1 polyclonal antibody | 1:500 |
| Rabbit Anti-Phospho-Bad (Ser136) polyclonal antibody | 1:500 |
| Rabbit Anti GSDMD-N monoclonal antibody | 1:1,000 |