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. 2021 Nov 5;9:740758. doi: 10.3389/fcell.2021.740758

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Copyright © 2021 Badanjak, Mulica, Smajic, Delcambre, Tranchevent, Diederich, Rauen, Schwamborn, Glaab, Cowley, Antony, Pereira, Venegas and Grünewald.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of cellular models used in the study. (A) Overview of the protocol used to derive microglia from iPSCs. (B) Immunostaining with Nanog, Sox-2 and Oct-4, coupled with nuclear staining with Hoechst, confirmed the iPSC identity of our cells. Scale bar, 50 μm. (C) Terminally differentiated microglia were confirmed to express the microglia-specific markers Iba1 and P2RY12. Scale bar, 30 μm. (D) Quantification of data from (C); no difference in Iba1 protein levels (Iba1 area was normalized for nuclei count); IPD, idiopathic PD; CTR, healthy control.