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. 2021 Nov 18;12:6741. doi: 10.1038/s41467-021-26904-6

Fig. 3. Muscle Mmp17 regulates crypts BMP signaling.

Fig. 3

a, b RNA seq assay comparing WT and Mmp17 KO crypts, we showed that loss of MMP17 in the muscle strongly affects epithelial gene expression to a greater extent than muscle gene expression. n = 3 biological replicates. c Graph shows the presence of genes related to SMAD4 signaling among the upregulated genes in WT vs KO crypts. d, e We observed higher SMAD4 signaling that was further confirmed by immunofluorescence in (d) (arrowheads indicate nuclear location in Mmp17 KO) and western blot (e). n = 3 mice/genotype. Scale bar 50 µm, 25 µm in magnified views to the right. f Representative confocal images showing pSmad 1/5/9 staining in intestinal crypts of WT vs KO mice. Arrowheads highlight crypt base pSmad 1/5/9 staining. Scale 50 µm. n = 3 independent experiments with 2–4 samples/genotype. Numerical data in (e) are means ± SD and were tested by one-way ANOVA (F = 7.43, p = 0.0106) followed by Tukey’s test (WT vs KO Crypts *p < 0.05). Source data are provided as a Source Data file.