Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 18;12:6708. doi: 10.1038/s41467-021-27039-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Location of a conserved motif in the glp-1 promoter of four Caenorhabditis species. Cbn C. brenneri, Cjp C. japonica, Cre C. remanei, Cel C. elegans. The conserved motif in C. elegans glp-1 promoter is highlighted in gray. Deleted bases in CRISPR-engineered glp-1 promoter mutant in red. b, c Immunofluorescence images (b) and quantification (c) of GLP-1::V5 protein in the distal germline of wild-type and glp-1prom∆ (5 bp deletion in conserved promoter motif) adult hermaphrodites. Data were expressed as mean ± s.e.m. and statistical significance was assessed by unpaired t-test. Scale bar = 10 µm. d Quantification of PZ nuclei of wild-type and glp-1prom∆ adult hermaphrodites. Data were expressed as mean ± s.e.m. and statistical significance was assessed by a two-tailed unpaired t-test. e Quantification of GLP-1::V5 protein in the distal germline of adult hermaphrodites (± sdn-1 RNAi) expressing wild-type glp-1 or glp-1prom∆ adult hermaphrodites. Data were expressed as mean ± s.e.m. and statistical significance was assessed by ordinary one-way ANOVA. f Quantification of PZ nuclei in the distal germline of adult hermaphrodites (± sdn-1 RNAi) expressing wild-type glp-1 or glp-1prom∆. Data were expressed as mean ± s.e.m. and statistical significance was assessed by ordinary one-way ANOVA. For all panels, n refers to the number of animals analyzed. P values as indicated. Source data are provided as a Source Data file.