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. 2021 Nov 18;12:6689. doi: 10.1038/s41467-021-26936-y

Fig. 5. MB002 cell line-specific peptides induce peptide-specific, poly-functional Class II-mediated T cell IFN-γ responses in vitro.

Fig. 5

Dendritic cells were loaded with MB002-specific peptides and co-cultured with non-adherent PBMCs. After three stimulations, peptide-specific T cell responses were analyzed by anti-IFN-γ ELISpot. a Summary data of IFN-γ response to pooled MB002 peptides in healthy donor 1. One-sided t-test was used to calculate the p-values, n = 3. p-values are indicated in the figure. b Summary data showing IFN-γ response to individual MB002 peptides in healthy Donor 2 (n = 2). c Summary data of MB002 and D556 TSATs populations and phenotypes (Error bars: mean + SD of five independent experiments). d Summary data showing CD4+ cytokine response to the pooled MB002 peptides and to the individual peptides 6 and 7 in the healthy donor 2. SFU: spot-forming units; 1 SFU = 1 T cell secreting IFN-γ; TSA T: Tumor-specific antigen T cell; a-MHC-I/II: anti-MHC Class I/II blocking antibodies; MB002 pep: pooled MB002 peptides; D556 pep: pooled D556 peptides; DMSO: dimethyl sulfoxide (peptide solvent; unstimulated control); actin: peptide specificity control; SEB: staphylococcus enterotoxin B (positive control). Gating strategy for intracellular staining (Supplementary Fig. 15). Gating strategy TSA T populations and phenotypes (Supplementary Fig. 16). Data are presented as mean values +/− SD. Source data are provided as a Source Data file.