Skip to main content
. 2021 Nov 18;12:6689. doi: 10.1038/s41467-021-26936-y

Fig. 6. MB002 TSA T cells specifically lyse partially HLA-matched tumor cells.

Fig. 6

To assess cytotoxic function, cryopreserved TSA T were thawed and rested overnight prior to plating with tumor targets. At the indicated time points, co-cultures were harvested and acquired as described in the “Methods”. a Representative dot plots from one healthy donor showing proliferation of tumor targets in the absence of TSA T (top row), moderate reduction of tumor targets in the presence of non-specifically activated T cells (PHA blasts; middle row), and robust lysis of tumor targets in the presence of TSA T (bottom row). Lysis was determined based on the disappearance of targets from quadrant 1 (red border). b Summary data of (a). NST non-specific T cells (PHA blasts). One-way ANOVA p-values are shown. Values at each time point were normalized to 0 h (100%). One-sided ANOVA test was used to calculate the p-values, n = 3. p-values are indicated in the figure. Gating strategy for expanded TSA T tumor cell cytotoxicity assays shown in Supplementary Fig. 18. Data are presented as mean values +/− SD. Source data are provided as a Source Data file.