Trajectory plots for NSC-Dulken. Trajectory plots were generated for redPATH (A), SCORPIUS (B), TSCAN (C), and Monocle2 (D), respectively, using the default functions in their respective R packages. For redPATH, the left panel shows the pseudotime progression of cells, while the right denotes the cell type information. For SCORPIUS, TSCAN, and Monocle2, the left panel shows the trajectory obtained from using the default gene selection method in their respective package. On the right, the input expression is pre-processed by using the GO selected genes from redPATH.