Fig. 2.
Post-treatment with iron did not reverse Tat-mediated increases in HIV-1 LTR transactivation: U87MG cells were incubated for 24 h with HIV-1 Tat protein (2 μg/ml) and CQ (100 μM) prior to the addition of and incubation for another 24 h with four types of iron A FeCl2, B FeCl3, C ammonium iron(III) citrate, and D iron(III) dextran at concentrations ranging from 10 to 50 μM. Post-treatment with iron did not significantly affect the ability of HIV-1 Tat to increase HIV-1 LTR transactivation. (n = 3; p > 0.05)