TABLE 1.
Reproducibility of serum TTV DNA levels by RTD-PCR assaya
Assay | Intra-assay reproducibility of TTV DNA level (log copies/ml)
|
Interassay reproducibility of TTV DNA level (log copies/ml)
|
||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Expt 1 | Expt 2 | Expt 3 | Expt 4 | Expt 5 | Mean | SD | CV (%) | Day 1 | Day 2 | Day 3 | Day 4 | Day 5 | Mean | SD | CV (%) | |
1 | 3.158 | 2.748 | 3.049 | 3.182 | 2.857 | 2.999 | 0.190 | 6.33 | 3.158 | 3.215 | 2.964 | 2.748 | 3.283 | 3.074 | 0.217 | 7.07 |
2 | 3.246 | 2.924 | 3.301 | 3.121 | 3.033 | 3.125 | 0.153 | 4.91 | 3.246 | 3.318 | 3.158 | 3.049 | 3.334 | 3.221 | 0.119 | 3.68 |
3 | 4.580 | 4.459 | 4.350 | 4.435 | 4.833 | 4.531 | 0.187 | 4.13 | 4.580 | 4.778 | 4.459 | 4.394 | 4.833 | 4.601 | 0.192 | 4.17 |
4 | 5.064 | 5.158 | 4.964 | 4.716 | 4.064 | 4.993 | 0.170 | 3.40 | 5.064 | 5.182 | 4.881 | 4.748 | 5.158 | 5.007 | 0.187 | 3.73 |
5 | 5.265 | 5.246 | 5.255 | 5.246 | 5.292 | 5.261 | 0.019 | 0.37 | 5.265 | 5.310 | 5.236 | 5.255 | 5.318 | 5.277 | 0.036 | 0.68 |
6 | 5.778 | 5.806 | 5.833 | 5.806 | 5.748 | 5.794 | 0.032 | 0.55 | 5.778 | 5.833 | 5.806 | 5.778 | 5.857 | 5.810 | 0.035 | 0.60 |
7 | NDb | ND | ND | ND | ND | ND | ND | ND | ND | ND | ||||||
8 | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
Primers and probe set B were used.
ND, not detected.