Skip to main content
. 2021 Nov 5;12:744454. doi: 10.3389/fimmu.2021.744454

Figure 2.

Figure 2

Intestinal ZIP-11 activates innate immunity against P. aeruginosa PA14 infection. (A) WT worms and zip-11(tm4554) worms were exposed to P. aeruginosa PA14 and scored for survival. (B) WT worms fed with vector control or zip-11 RNAi bacteria were exposed to P. aeruginosa PA14 and scored for survival. (C) High magnification images of zip-11p::zip-11::SL2::GFP transgenic worms show zip-11 is expressed in intestine, pharynx, and hypodermis. Scale bar: 20 μm. (D–H) Tissue-specific RNAi worm strains including TU3311/neuron (D), NR222/hypodermis (E), WM118/muscle (F), DCL569/germline (G) and VP303/intestine (H) fed with vector control or zip-11 RNAi bacteria were exposed to P. aeruginosa PA14 and scored for survival. (I) WT, zip-11(tm4554), zip-11(tm4554);aijEx179[zip-11p::zip-11] and zip-11(tm4554);aijEx180[ges-1p::zip-11] worms were exposed to P. aeruginosa PA14 and scored for survival. Statistical significance was determined by log-rank test for killing assays. Please see Table S4 in Supplementary Material for detailed statistical analysis of killing assay data. ***p < 0.001; n.s., not significant.